期刊
REDOX BIOLOGY
卷 46, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.redox.2021.102075
关键词
AhpC1; P. aeruginosa (PA14); Virulence; Inflammation; Oxidants; Urate hydroperoxide
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2013/07937-8, 2018/14898-2, 2015/10411-3]
- Fundacao de Amparo a Pesquisa da Bahia (FAPESB) [RED038/2014]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [462401/2014-6]
- UESB
- FAPESP [2019/26473-9, 2015/21563-9]
- FAPESB [BOL1270/2017]
The study identified the importance of 2-Cys peroxiredoxin in Pseudomonas aeruginosa in neutralizing oxidants and evading host immune system, with the deletion of AhpC1 leading to increased sensitivity to oxidants and lower virulence in mice infection. The enzyme plays a crucial role in detoxifying inflammatory organic peroxides like urate hydroperoxide.
Pseudomonas aeruginosa is an opportunistic bacterium in patients with cystic fibrosis and hospital acquired infections. It presents a plethora of virulence factors and antioxidant enzymes that help to subvert the immune system. In this study, we identified the 2-Cys peroxiredoxin, alkyl-hydroperoxide reductase C1 (AhpC1), as a relevant scavenger of oxidants generated during inflammatory oxidative burst and a mechanism of P. aeruginosa (PA14) escaping from killing. Deletion of AhpC1 led to a higher sensitivity to hypochlorous acid (HOCl, IC50 3.2 +/- 0.3 versus 19.1 +/- 0.2 mu M), hydrogen peroxide (IC50 91.2 +/- 0.3 versus 496.5 +/- 6.4 mu M) and the organic peroxide urate hydroperoxide. Delta ahpC1 strain was more sensitive to the killing by isolated neutrophils and less virulent in a mice model of infection. All mice intranasally instilled with Delta ahpC1 survived as long as they were monitored (15 days), whereas 100% wild-type and Delta ahpC1 complemented with ahpC1 gene (Delta ahpC1 attB:ahpC1) died within 3 days. A significantly lower number of colonies was detected in the lung and spleen of Delta ahpC1-infected mice. Total leucocytes, neutrophils, myeloperoxidase activity, pro-inflammatory cytokines, nitrite production and lipid peroxidation were much lower in lungs or bronchoalveolar liquid of mice infected with Delta ahpC1. Purified AhpC neutralized the inflammatory organic peroxide, urate hydroperoxide, at a rate constant of 2.3 +/- 0.1 x 10(6) M(-1)s(-1), and only the Delta ahpC1 strain was sensitive to this oxidant. Incubation of neutrophils with uric acid, the urate hydroperoxide precursor, impaired neutrophil killing of wild-type but improved the killing of Delta ahpC1. Hyperuricemic mice presented higher levels of serum cytokines and succumbed much faster to PA14 infection when compared to normouricemic mice. In summary, Delta ahpC1 PA14 presented a lower virulence, which was attributed to a poorer ability to neutralize the oxidants generated by inflammatory oxidative burst, leading to a more efficient killing by the host. The enzyme is particularly relevant in detoxifying the newly reported inflammatory organic peroxide, urate hydroperoxide.
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