期刊
JOURNAL OF SYNCHROTRON RADIATION
卷 29, 期 -, 页码 581-590出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1600577522000984
关键词
microcrystallography; macromolecular crystallography; MD3Up high-precision multi-axis diffractometer
ID23-2 is a beamline at ESRF dedicated to macromolecular crystallography. Its recent upgrades include the use of compound refractive lenses and multilayer mirrors for intense focused microbeam and the improvement of sample environment with advanced sample changer/storage system and high-precision multi-axis diffractometer. It also provides automatic data reduction and analysis for advanced protocols.
ID23-2 is a fixed-energy (14.2 keV) microfocus beamline at the European Synchrotron Radiation Facility (ESRF) dedicated to macromolecular crystallography. The optics and sample environment have recently been redesigned and rebuilt to take full advantage of the upgrade of the ESRF to the fourth generation Extremely Brilliant Source (ESRF-EBS). The upgraded beamline now makes use of two sets of compound refractive lenses and multilayer mirrors to obtain a highly intense (>10(13) photons s(-1)) focused microbeam (minimum size 1.5 mu m x 3 mu m full width at half-maximum). The sample environment now includes a FLEX-HCD sample changer/storage system, as well as a state-of-the-art MD3Up high-precision multi-axis diffractometer. Automatic data reduction and analysis are also provided for more advanced protocols such as synchrotron serial crystallographic experiments.
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