4.3 Article

Co-flow injection for serial crystallography at X-ray free-electron lasers

期刊

JOURNAL OF APPLIED CRYSTALLOGRAPHY
卷 55, 期 -, 页码 1-13

出版社

INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1600576721011079

关键词

microfluidic devices; serial crystallography; 3D printing; X-ray free-electron lasers; XFELs; viscous media; sample consumption

资金

  1. STC Program of the US National Science Foundation through BioXFEL [1231306]
  2. NSF ABI Innovations [1565180]
  3. NSF CAREER [1943448]
  4. NSF MCB [1817862]
  5. National Institutes of Health [R01GM095583, S10OD021816-01, R01GM120537]
  6. US Department of Energy, Office of Science, Basic Energy Sciences [DE-SC0002164]
  7. NSF [1231306]
  8. Biodesign Center for Applied Structural Discovery at Arizona State University
  9. Cluster of Excellence 'CUI: Advanced Imaging of Matter' of the Deutsche Forschungsgemeinschaft (DFG) (EXC 2056) [390715994]
  10. Div Of Biological Infrastructure
  11. Direct For Biological Sciences [1943448] Funding Source: National Science Foundation
  12. Div Of Molecular and Cellular Bioscience
  13. Direct For Biological Sciences [1817862] Funding Source: National Science Foundation

向作者/读者索取更多资源

This study proposes a new method to address the issue of crystal introduction in the X-ray beam. By using an inert oil phase to supplement the flow of the sample, stable injection of crystals from two different materials can be achieved, avoiding crystal clogging and potentially increasing the preservation rate of crystal samples, enabling degradation-free light-induced time-resolved SFX.
Serial femtosecond crystallography (SFX) is a powerful technique that exploits X-ray free-electron lasers to determine the structure of macromolecules at room temperature. Despite the impressive exposition of structural details with this novel crystallographic approach, the methods currently available to introduce crystals into the path of the X-ray beam sometimes exhibit serious drawbacks. Samples requiring liquid injection of crystal slurries consume large quantities of crystals (at times up to a gram of protein per data set), may not be compatible with vacuum configurations on beamlines or provide a high background due to additional sheathing liquids present during the injection. Proposed and characterized here is the use of an immiscible inert oil phase to supplement the flow of sample in a hybrid microfluidic 3D-printed co-flow device. Co-flow generation is reported with sample and oil phases flowing in parallel, resulting in stable injection conditions for two different resin materials experimentally. A numerical model is presented that adequately predicts these flow-rate conditions. The co-flow generating devices reduce crystal clogging effects, have the potential to conserve protein crystal samples up to 95% and will allow degradation-free light-induced time-resolved SFX.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.3
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据