期刊
FRONTIERS IN IMMUNOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2022.812514
关键词
SARS-CoV-2; COVID-19; asymptomatic infection; disease severity; viral persistence; singlecell RNA sequencing (scRNA-seq)
类别
This study identified distinct cell subsets associated with different clinical presentations of COVID-19, providing insights into the immunopathogenesis of the disease. Classical monocytes and pDC were found to be correlated with disease severity and viral persistence.
The cell-mediated protective and pathogenic immune responses to SARS-CoV-2 infection remain largely elusive. Here we identified 76 distinct cell subsets in the PBMC samples that were associated with various clinical presentations of COVID-19 using scRNA-seq technology coupled with a deep and comprehensive analysis of unique cell surface markers and differentially expressed genes. We revealed that (TRAV1-2(+)CD8(+))MAIT cells and (NCAM1(hi)CD160(+))NK cells significantly enriched in the asymptomatic subjects whereas (LAG3(+)CD160(+)CD8(+))NKT cells increased in the symptomatic patients. We also observed that (CD68(-)CSF1R(-)IL1B(hi)CD14(+))classical monocytes were positively correlated with the disease severity. Moreover, (CD33(-)HLA-DMA(-)CD14(+))classical monocytes and (CLEC10A(-)S100A9(lo))pDC were associated with the viral persistence. The GO and KEGG analyses identified enriched pathways related to immune responses, inflammation, and apoptosis. These findings may enhance our understanding of the immunopathogenesis of COVID-19 and help develop novel strategies against SARS-CoV-2 infection.
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