期刊
FRONTIERS IN IMMUNOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2021.732756
关键词
SARS-CoV-2; diagnostics; RT-PCR; LAMP; biosensors; point of care; CRISPR-Cas
类别
资金
- Science and Engineering Research Board-Intensification of Research in High Priority Area (SERB-IRHPA) [IPA/2020/000069]
- Slovenian Research Agency (ARRS) [P1-0143]
- DST, New Delhi [IF180729]
A vaccine is crucial, but rapid and precise diagnostic methods are also essential in monitoring and combating the spread of SARS-CoV-2 and its variants. A variety of conventional and modern diagnostic methods have been developed, with ongoing research focusing on developing more economical tools to tackle the COVID-19 pandemic.
Coronavirus disease 2019 (COVID-19), which started out as an outbreak of pneumonia, has now turned into a pandemic due to its rapid transmission. Besides developing a vaccine, rapid, accurate, and cost-effective diagnosis is essential for monitoring and combating the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its related variants on time with precision and accuracy. Currently, the gold standard for detection of SARS-CoV-2 is Reverse Transcription Polymerase Chain Reaction (RT-PCR), but it lacks accuracy, is time-consuming and cumbersome, and fails to detect multi-variant forms of the virus. Herein, we have summarized conventional diagnostic methods such as Chest-CT (Computed Tomography), RT-PCR, Loop Mediated Isothermal Amplification (LAMP), Reverse Transcription-LAMP (RT-LAMP), as well new modern diagnostics such as CRISPR-Cas-based assays, Surface Enhanced Raman Spectroscopy (SERS), Lateral Flow Assays (LFA), Graphene-Field Effect Transistor (GraFET), electrochemical sensors, immunosensors, antisense oligonucleotides (ASOs)-based assays, and microarrays for SARS-CoV-2 detection. This review will also provide an insight into an ongoing research and the possibility of developing more economical tools to tackle the COVID-19 pandemic.
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