期刊
FRONTIERS IN IMMUNOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2022.752189
关键词
systemic lupus erythematosus; age-associated B cells; ribonuclease A family member 2; interleukin 10; monocytes
类别
资金
- National Natural Science Foundation of China [81971517, 81771745]
- Jiangsu Provincial Medical Talent Program [ZDRCA2016059]
- Key Program of National Natural Science Foundation of China [81930043]
- Major International (Regional) Joint Research Project of China [81720108020]
Systemic lupus erythematosus (SLE) is characterized by the production of pathogenic autoantibodies. This study found that Ribonuclease A family member 2 (RNase2) is highly expressed in SLE patients and is associated with the increase of autoreactive B cells known as age-associated B cells (ABCs). Reduction of RNASE2 expression led to a decrease in ABCs, accompanied by a reduction in total IgG and IL-10 levels. It was also found that both RNASE2 and IL-10 in SLE patients were mainly derived from monocytes. The study concluded that RNASE2 might induce the production of ABCs via IL-10 secreted from monocytes, contributing to the pathogenesis of SLE.
Systemic lupus erythematosus (SLE) is characterized by the production of pathogenic autoantibodies. Ribonuclease A family member 2 (RNase2) is known to have antiviral activity and immunomodulatory function. Although RNASE2 level has been reported to be elevated in SLE patients based on mRNA microarray detection, its pathologic mechanism remains unclear. Here, we confirmed that RNASE2 was highly expressed in PBMCs from SLE patients and associated with the proportion of CD11c(+)T-bet(+) B cells, a class of autoreactive B cells also known as age-associated B cells (ABCs). We showed that reduction of RNASE2 expression by small interfering RNA led to the decrease of ABCs in vitro, accompanied by total IgG and IL-10 reduction. In addition, we demonstrated that both RNASE2 and IL-10 in peripheral blood of lupus patients were mainly derived from monocytes. RNASE2 silencing in monocytes down-regulated IL-10 production and consequently reduced ABCs numbers in monocyte-B cell co-cultures, which could be restored by the addition of recombinant IL-10. Based on above findings, we concluded that RNASE2 might induce the production of ABCs via IL-10 secreted from monocytes, thus contributing to the pathogenesis of SLE.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据