期刊
BIOENGINEERED
卷 13, 期 2, 页码 4226-4234出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/21655979.2022.2027174
关键词
Circ-CLK1; oral squamous cell carcinoma; apoptosis
This study found that circ-CLK1 is significantly upregulated in oral squamous cell carcinoma and inhibiting circ-CLK1 can reduce cell viability and proliferation while promoting apoptosis. Furthermore, circ-CLK1 inhibits apoptosis through the miR-18b-5p/YBX2 axis.
This study aimed to explore the role of circular-CDC like kinase 1 (circ-CLK1) in the pathogenesis of oral squamous cell carcinoma (OSCC). Circ-CLK1 expression levels were detected via reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The effects of circ-CLK1 knockdown on the viability and apoptosis of OSCC cells were determined using the cell counting kit-8 (CCK-8) assay, EdU staining, flow cytometry, and Western blotting. StarBase and TargetScan were used to predict targeting relationships, which were then confirmed by the dual luciferase reporter assay and RNA pull-down assay. We found that the expression of circ-CLK1 was significantly higher in OSCC patients and cell lines. Inhibition of circ-CLK1 reduced the viability and proliferation of OSCC cells while enhancing their apoptosis. However, inhibiting miR-18b-5p or overexpression of Y-box protein 2 (YBX2) can reverse the effect of circ-CLK1 knockdown on OSCC cells. Therefore, circ-CLK1 inhibited the apoptosis of OSCC cells through the miR-18b-5p/YBX2 axis, and these findings suggest that circ-CLK1 could be a potential therapeutic target for OSCC patients.
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