期刊
BIOENGINEERED
卷 12, 期 2, 页码 12954-12964出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/21655979.2021.2012949
关键词
Imperatorin; knee osteoarthritis; synovial fibrosis; hypoxia; synovitis; NLR family pyrin domain-containing 3 inflammasome
资金
- National Science Fund of China [81774334]
- Leading Talents of Traditional Chinese Medicine Project [SLJ0207]
The study demonstrated that imperatorin can reduce synovial inflammation and fibrosis, inhibit production of inflammatory mediators and cytokines, and decrease expressions of factors promoting synovial fibrosis.
We aimed to clarify the therapeutic effects of imperatorin (IMP) on knee osteoarthritis (KOA). Thirty 3-month-old SD male rats were randomly divided into Normal, monosodium iodoacetate (MIA) and MIA+IMP groups. Their synovial tissues were subjected to histopathological analysis. Primary synovial fibroblasts obtained from additional normal rats were treated by lipopolysaccharide (LPS) and then IMP. The mRNA and protein expressions of factors related to synovitis and synovial fibrosis were detected by qRT-PCR and Western blotting, respectively. The concentrations of inflammatory factors IL-1 beta and IL-18 were measured by ELISA. IMP reduced HIF-1 alpha, NLR family pyrin domain-containing 3 inflammasome expression and IL-1 beta, IL-18 production in synovial fibroblasts induced by LPS. IMP also downregulated synovial fibrosis markers. In vitro study revealed that MIA-induced synovitis and synovial fibrosis were relieved by IMP. IMP relieves the inflammation associated with synovitis and synovial fibrosis. It reduces the production of pro-inflammatory mediators and cytokines and inhibits TGF-beta 1, TIMP-1 and VEGF expressions that promote synovial fibrosis.
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