4.7 Article

lncRNA DANCR promotes the migration an invasion and of trophoblast cells through microRNA-214-5p in preeclampsia

期刊

BIOENGINEERED
卷 12, 期 2, 页码 9424-9434

出版社

TAYLOR & FRANCIS INC
DOI: 10.1080/21655979.2021.1988373

关键词

Chorionic trophoblast cells; miR-214-5p; epithelial-mesenchymal transition; lncRNA DANCR; preeclampsia

资金

  1. Joint Construction Project of Henan Medical Science and Technology Research Plan in 2020 [LHGJ20200017]

向作者/读者索取更多资源

Studies have shown that lncRNA DANCR is down-regulated in placental tissues of patients with preeclampsia, and manipulating its expression can significantly alter trophoblast cell functions. Furthermore, the interaction between lncRNA DANCR and miR-214-5p was confirmed, along with the activation of the PI3K/AKT signaling pathway to promote cell migration and invasion. This suggests a potential new target for therapy in preeclampsia.
Studies have shown that lncRNA DANCR is down-regulated in placental tissues of patients with preeclampsia (PE). The aim of this study was to explore the effect of lncRNA DANCR on trophoblast cells as well as its acting mechanism. We disrupted or overexpressed lncRNA DANCR in trophoblast cells HTR-8/SVneo and JEG-3 and detected the associated cellular functional changes by MTT, flow cytometry, Transwell experiment, and scratch experiment. The results showed that overexpression of lncRNA DANCR significantly increased the proliferation, invasion, migration, and EMT process of trophoblast cells. Interfering with lncRNA DANCR showed the opposite result. Further, the targeted interaction between lncRNA DANCR and miR-214-5p was confirmed by the dual-luciferase reporter gene assay. In addition, the expression of PI3K/AKT signaling pathway-related proteins was analyzed by Western blot. Overexpression of lncRNA DANCR can increase the phosphorylation of PI3K/AKT protein and activate this signaling pathway. In conclusion, the enforcing of lncRNA DANCR activates the activation of the PI3K/AKT pathway by down-regulating miR-214-5p, and promotes the migration and invasion of chorionic trophoblast cells. This provides a potential new target for PE therapy.

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