期刊
BIOENGINEERED
卷 13, 期 1, 页码 1185-1197出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/21655979.2021.2017579
关键词
Hepatocellular carcinoma; Has_circ_0008583; miR-1301-3p; METTL3
资金
- Natural Science Foundation of Jiangxi, China [20202BABL206018]
- National Natural Science Foundation of China [81960440, 82070594]
- Financial Support Program for Academic Leaders of Major Disciplines in Jiangxi Province, China [20182BCB22018]
The study found that hsa_circ_0008583 promotes the progression of hepatocellular carcinoma through the miR-1301-3p/METTL3 axis, specifically by promoting cell proliferation, migration, and invasion.
Has_circ_0008583 is reported to be involved in the progression of hepatocellular carcinoma (HCC), while its biological role in HCC remains unclear. Here, the qRT-PCR was used to detect the expression of has_circ_0008583. The CCK-8 kit was performed to measure cell proliferation. The cell migration and invasion were evaluated by Transwell. A dual-luciferase reporter assay was performed to confirm the target combination between the genes in has_circ_0008583/miR-1301-3p/METTL3 axis. The in vivo role of has_circ_0008583 was verified by murine xenograft assay. Our data showed that hsa_circ_0008583 was upregulated in HCC tissues and cells. Hsa_circ_0008583 overexpression promoted Hep3B cell proliferation, migration and invasion, but hsa_circ_0008583 silencing had an opposing influence. MiR-1301-3p is directly bound to hsa_circ_0008583 and METTL3. MiR-1301-3p overexpression or METTL3 knockdown could partially counteract hsa_circ_0008583 overexpression-mediated influence on HCC cell behaviors. In addition, hsa_circ_0008583 depletion inhibits HCC tumor growth in vivo. In conclusion, hsa_circ_0008583 promotes HCC progression through the miR-1301-3p/METTL3 axis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据