4.6 Article

Biophysical and Biomechanical Effect of Low Intensity US Treatments on Pancreatic Adenocarcinoma 3D Cultures

期刊

APPLIED SCIENCES-BASEL
卷 12, 期 2, 页码 -

出版社

MDPI
DOI: 10.3390/app12020666

关键词

biological tissue; cancer therapy; LICUS; LIPUS; living cells; spheroids; ultrasound stimulation; controlled power wideband amplifier

资金

  1. Associazione Italiana per la Ricerca sul Cancro (AIRC) [IG 21510]
  2. PRIN Italian Ministry of University and Research (MIUR) Leveraging basic knowledge of ion channel network in cancer for innovative therapeutic strategies (LIONESS) [20174TB8KW]
  3. European Union [813834]
  4. AIRC fellowship for Italy [24020]
  5. Ente Cassa di Risparmio di Firenze

向作者/读者索取更多资源

Current advancements in medical technology focus on targeted therapies that minimize side effects on healthy tissues. In cancer research, ultrasound has shown potential in inhibiting cancer cell proliferation while preserving healthy cells. However, there are still unknown aspects of low intensity ultrasound treatment that need further exploration.
Current developments in medical technology have focused on therapeutic treatments that selectively and effectively address specific pathological areas, minimizing side effects on healthy tissues. In this regard, many procedures have been developed to provide non-invasive therapy, for example therapeutic ultrasound (US). In the medical field, in particular in cancer research, it has been observed how ultrasounds can cause cell death and inhibit cell proliferation of cancer cells, while preserving healthy ones with almost negligible side effects. Various studies have shown that low intensity pulse ultrasound (LIPUS) and low intensity continuous ultrasound (LICUS) regulate the proliferation, cell differentiation and cavitation phenomena. Nowadays, there are poorly known aspects of low intensity US treatment, in terms of biophysical and biomechanical effects on target cells. The aim of this study is to set up an innovative apparatus for US treatment of pancreatic ductal adenocarcinoma (PDAC) cells, monitoring parameters such as acoustic intensity, acoustic pressure, stimulation frequency and treatment protocol. To this purpose, we have developed a custom-made set up for the US stimulation at 1.2 and 3 MHz of tridimensional (3D) cultures of PDAC cells (PANC-1, Mia Paca-2 and BxPc3 cells). Images of the 3D cultures were acquired, and the Calcein/PI assay was applied to detect US-induced cell death. Overall, the setup we have presented paves the way to an innovative protocol for tumor treatment. The system can be used either alone or in combination with small molecules or recombinant antibodies in order to propose a novel combined therapeutic approach.

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