iEnhancer-Deep: A Computational Predictor for Enhancer Sites and Their Strength Using Deep Learning

Enhancers are short motifs that contain high position variability and free scattering. Identifying these non-coding DNA fragments and their strength is vital because they play an important role in the control of gene regulation. Enhancer identification is more complicated than other genetic factors due to free scattering and their very high amount of locational variation. To classify this biological difficulty, several computational tools in bioinformatics have been created over the last few years as current learning models are still lacking. To overcome these limitations, we introduce iEnhancer-Deep, a deep learning-based framework that uses One-Hot Encoding and a convolutional neural network for model construction, primarily for the identification of enhancers and secondarily for the classification of their strength. Parallels between the iEnhancer-Deep and existing state-of-the-art methodologies were drawn to evaluate the performance of the proposed model. Furthermore, a cross-species test was carried out to assess the generalizability of the proposed model. In general, the results show that the proposed model produced comparable results with the state-of-the-art models.

Automated summary

Enhancers are short motifs with high position variability and play an important role in gene regulation. Identification of enhancers is challenging due to their complexity, but recent advancements in computational tools and deep learning frameworks have shown comparable results with state-of-the-art methodologies.