期刊
AMB EXPRESS
卷 11, 期 1, 页码 -出版社
SPRINGER
DOI: 10.1186/s13568-021-01339-w
关键词
Oxygen consumption rate; Pesticide degradation; Respiratory quotient; Rhizobacteria
This study aimed to evaluate the growth, respiratory activity, and biodegradation of chlorpyrifos in cultures of Azotobacter vinelandii ATCC 12837. By modifying culture conditions, the strain showed tolerance and degradation ability towards chlorpyrifos, with particularly good growth and degradation performance in liquid medium containing the pesticide.
This study aimed to evaluate the growth, respiratory activity, and biodegradation of chlorpyrifos in cultures of Azotobacter vinelandii ATCC 12837. A strategy based on the modification of culture media and aeration conditions was carried out to increase the cell concentration of A. vinelandii, in order to favor and determine its tolerance to chlorpyrifos and its degradation ability. The culture in shaken flasks, using sucrose as a carbon source, significantly improved the growth compared to media with mannitol. When the strain was cultivated under oxygen-limited (5.5, 11.25 mmol L-1 h(-1)) and no-oxygen-limited conditions (22 mmol L-1 h(-1)), the growth parameters were not affected. In cultures in a liquid medium with chlorpyrifos, the bacteria tolerated a high pesticide concentration (500 ppm) and the growth parameters were improved even under conditions with a reduced carbon source (sucrose 2 g L-1). The strain degraded 99.6% of chlorpyrifos at 60 h of cultivation, in co-metabolism with sucrose; notably, A. vinelandii ATCC 12837 reduced by 50% the initial pesticide concentration in only 6 h (DT50).
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