Best practices for time-resolved serial synchrotron crystallography

With recent developments in X-ray sources, instrumentation and data-analysis tools, time-resolved crystallographic experiments, which were originally the preserve of a few expert groups, are becoming simpler and can be carried out at more radiation sources, and are thus increasingly accessible to a growing user base. However, these experiments are just that: discrete experiments, not just 'data collections'. As such, careful planning and consideration of potential pitfalls is required to enable a successful experiment. Here, some of the key factors that should be considered during the planning and execution of a time-resolved structural study are outlined, with a particular focus on synchrotron-based experiments.

Automated summary

With advancements in X-ray sources, equipment, and data-analysis tools, time-resolved crystallographic experiments are becoming more accessible to a wider user base. This article highlights the key factors that need to be considered when planning and conducting a time-resolved structural study, with a focus on synchrotron-based experiments.