4.2 Article

Structural insight into DNA recognition by bacterial transcriptional regulators of the SorC/DeoR family

期刊

出版社

INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S2059798321009633

关键词

SorC/DeoR family; protein-DNA complexes; transcription repressors; Bacillus subtilis; deoxyribonucleoside regulator bsDeoR; gene regulator bsCggR; carbohydrate catabolism

资金

  1. European Regional Development Fund
  2. OP RDE [CZ.02.1.01/0.0/0.0/16_019/0000729]

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The SorC/DeoR family is a large family of bacterial transcription regulators involved in carbohydrate metabolism and quorum sensing. Structural studies of two members from Bacillus subtilis revealed the structural basis of DNA recognition and proposed a common mode of DNA recognition likely shared by other family members.
The SorC/DeoR family is a large family of bacterial transcription regulators that are involved in the control of carbohydrate metabolism and quorum sensing. To understand the structural basis of DNA recognition, structural studies of two functionally characterized SorC/DeoR family members from Bacillus subtilis were performed: the deoxyribonucleoside regulator bsDeoR and the central glycolytic genes regulator bsCggR. Each selected protein represents one of the subgroups that are recognized within the family. Crystal structures were determined of the N-terminal DNA-binding domains of bsDeoR and bsCggR in complex with DNA duplexes degrees representing the minimal operator sequence at resolutions of 2.3 and 2.1 angstrom, respectively. While bsDeoR(DBD) contains a homeodomain-like HTH-type domain, bsCggR(DBD) contains a winged helix- turn-helix-type motif. Both proteins form C2-symmetric dimers that recognize two consecutive major grooves, and the protein-DNA interactions have been analyzed in detail. The crystal structures were used to model the interactions of the proteins with the full DNA operators, and a common mode of DNA recognition is proposed that is most likely to be shared by other members of the SorC/DeoR family.

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