One-pot microbial bioconversion of wheat bran ferulic acid to biovanillin

Due to growing consumer preference towards natural ingredients in food products, the production of flavors by microbial biotransformation of agrowastes provides an eco-friendly, cost-effective and sustainable pathway for biovanillin production. In the present study, biovanillin was produced by microbial biotransformation of ferulic acid (FA) using Streptomyces sp. ssr-198. The strain was able to grow in glucose medium supplemented with 1 g/L FA and produce 20.91 +/- 1.11 mg/L vanillin within 96 h, along with 5.78 +/- 0.13 mg/L vanillic acid in 144 h. Estimation of enzymes involved in FA degradation detected maximum feruloyl-CoA synthetase activity (1.21 +/- 0.03 U/mg protein) at 96 h and maximum vanillin dehydrogenase activity (0.31 +/- 0.008 U/mg protein) at 168 h, with small amounts of ferulic acid esterase activity (0.13 +/- 0.002 U/mg protein) in the fermentation medium. Further, the glucose deficient production medium supplemented with 3 g/L of ferulic acid when inoculated with Streptomyces sp. ssr-198 (6% wet weight) produced maximum vanillin (685 +/- 20.11 mg/L) within 72 h at 37 degrees C under agitation (150 rpm) and declined thereafter. Furthermore, in a one-pot experiment, wherein crude ferulic acid esterase (700 IU/g of substrate) from Enterococcus lactis SR1 was added into 10% w/v wheat bran (natural source of ferulic acid) based medium and was inoculated with 1% w/v of Streptomyces sp. ssr-198 resulted in maximum vanillin production (1.02 +/- 0.02 mg/g of substrate) within 60 h of incubation. The study provides an insight into synergistic effect of using FAE of E. lactis SR1 and Streptomyces sp. ssr-198 for bioproduction of biovanillin using agro residues.

Automated summary

This study focuses on the production of biovanillin through microbial biotransformation of agrowastes using Streptomyces sp. ssr-198. The strain was able to produce vanillin and vanillic acid, with varying enzyme activities and production levels under different culture conditions. Additionally, a synergistic effect was observed when using ferulic acid esterase from Enterococcus lactis SR1 in combination with Streptomyces sp. ssr-198 for biovanillin production using agro residues.