Anti-Biofilm, Antioxidant and Cytotoxic Potential of F5, a Peptide Derived from Waste Generated During the Processing of the White Shrimp, Metapenaeus monoceros (Fabricius, 1798)

In previous research works, we have described the production of 7 kDa F5 peptide after a hydrolysis of the white shrimp, Metapenaeus monoceros (Fabricius, 1798) by-product, using a serine alkaline protease (SPVP) purified from Aeribacillus pallidus strain VP3. The present study aims to explore the antioxidative potentials of F5 peptide by both in vitro and in vivo assays. The anti-biofilm activity was performed, showing 50% inhibition at 2 mu g/mL, 3 mu g/mL, 19 mu g/mL, and 45 mu g/mL for Staphylococcus aureus, Escherichia coli, Bacillus cereus, and Pseudomonas aeruginosa, respectively. Consistently, the antioxidative capacity was tested in vitro against 2,2'-azino-bis-(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS) cation radical, beta-caroten-linoleic acid bleaching, chelating capacity of ferrous ion, and ferric reducing power assays. The cytotoxic effects of F5 peptide on the Human embryonic kidney HEK293 cells were subsequently tested. Remarkably, the F5 peptide was able to improve significantly HEK293 cell viability. To further assess this bioactivity, an in vivo study was performed on adult mice models. The animals were divided into three groups: controls, 100 mg and 200 mg of F5/kg per bodyweight. Indeed, the F5 peptide could prevent the lipid and protein oxidation damage in kidney cells, improving the antioxidative enzymatic and non-enzymatic capacities.

Automated summary

The research demonstrates that the F5 peptide exhibits strong anti-biofilm activity and antioxidative potential, showing significant antioxidative capacity in vitro and improving cell viability in HEK293 cells. In vivo studies further confirm that F5 peptide can prevent lipid and protein oxidation damage in kidney cells.