期刊
PEERJ
卷 9, 期 -, 页码 -出版社
PEERJ INC
DOI: 10.7717/peerj.12596
关键词
Theileria spp; Polymerase chain reaction (PCR); 18S rRNA
资金
- King Saud University, Riyadh, Saudi Arabia [RSP-2021/99]
The study found that Theileria spp. were detected in 57.8% of sheep and 51.9% of goats in Jeddah, Saudi Arabia. Phylogenetic analysis of the sequence data revealed that the T. ovis identified in this study were closely related to an isolate from Turkey, with pairwise identity ranging from 84.4% to 99.8% and coverage ranging from 52.35% to 99.79%.
Background. Thileriosis is a tick-born disease caused by hemoprotozoan parasites which has global veterinary and economic implications. Methods. Blood samples were collected from 216 sheep and 83 goats from Jeddah, Saudi Arabia, were analyzed to determine whether the animals were infected with Theileria spp. parasites. The parasites were detected using a polymerase chain reaction (PCR) targeting the gene of 18S rRNA followed by sequencing. Results. According to obtained findings, Theileria spp. were detected in sheep (57.8%, 48/83) and goats (51.9%, 112/216). Phylogenetic analysis to sequence data showed that T. ovis identified in this study were found to be closely connected to an isolate from Turkey, with 84.4-99.8% pairwise identity and 52.35-99.79% coverage.
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