期刊
PEERJ
卷 9, 期 -, 页码 -出版社
PEERJ INC
DOI: 10.7717/peerj.12513
关键词
Oplopanax elatus; Intestinal microbiota; UPLC-Q-TOF/MS; Metabolic profiles; Biotransformation
资金
- National Natural Science Foundation of China [81803970]
- NIH/NCCAM [AT004418, AT005362]
- Young Talent Promotion Project of China Association of Chinese Medicine [CACM-2019-QNRC2A01]
- Young Scientists Development Program of Beijing University of Chinese Medicine [2020-JYB-XJSJJ-003]
- Fundamental Research Funds for the Central Universities
The study identified chemical components and metabolites of Oplopanax elatus root bark extract using UPLC-Q-TOF/MS, finding two polyynes as the main components and proposing various metabolic pathways for generating metabolites. It suggested that intestinal microbiota may play a crucial role in mediating the bioactivity of Oplopanax elatus.
Oplopanax elatus (Nakai) Nakai, in the Araliaceae family, has been used in traditional Chinese medicine (TCM) to treat diseases as an adaptogen for thousands of years. This study established an ultra-performance liquid chromatography coupled with quadrupole time-of -flight tandem mass spectrometry (UPLC-Q-TOF/MS) method to identify chemical components and biotransformation metabolites of root bark extract from O. elatus. A total of 18 compounds were characterized in O. elatus extract, and 62 metabolites by human intestinal microbiota were detected. Two polyynes, falcarindiol and oplopandiol were recognized as the main components of O. elatus, whose metabolites are further illustrated. Several metabolic pathways were proposed to generate the detected metabolites, including methylation, hydrogenation, demethylation, dehydroxylation, and hydroxylation. These findings indicated that intestinal microbiota might play an essential role in mediating the bioactivity of O. elatus.
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