期刊
NANOMATERIALS
卷 11, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/nano11123433
关键词
psoriasis; methotrexate; Eudragit E100; nanoparticles; chitosan; hydrogel; in vitro release; imiquimod induced psoriasis model
The study developed a nanoparticle-loaded chitosan hydrogel with Methotrexate (MTX) for treating psoriasis in a mouse model, demonstrating its potential effectiveness in the treatment of psoriasis.
Methotrexate (MTX), the gold standard against psoriasis, poses severe problems when administered systemically viz increased toxicity, poor solubility and adverse reactions. Hence, a topical formulation of MTX for the management of psoriasis can be an effective approach. The present study aimed to develop an MTX based nanoparticle-loaded chitosan hydrogel for evaluating its potential efficacy in an imiquimod-induced psoriatic mice model. MTX-NPs loaded hydrogel was prepared and optimized using the o/w emulsion solvent evaporation method. Particle size, zeta potential, entrapment efficiency, in vitro drug release, ex vivo permeation, skin irritation and deposition studies were performed. Psoriatic Area and Severity Index (PASI) score/histopathological examinations were conducted to check the antipsoriatic potential of MTX-NPs loaded hydrogel using an imiquimod (IMQ)-induced psoriatic model. Optimized MTX-NPs showed a particle size of 256.4 +/- 2.17 nm and encapsulation efficiency of 86 +/- 0.03%. MTX-NPs loaded hydrogel displayed a 73 +/- 1.21% sustained drug release in 48 h. Ex vivo permeation study showed only 19.95 +/- 1.04 mu g/cm(2) of drug permeated though skin in 24 h, while epidermis retained 81.33% of the drug. A significant decrease in PASI score with improvement to normalcy of mice skin was observed. The developed MTX-NPs hydrogel displayed negligible signs of mild hyperkeratosis and parakeratosis, while histopathological studies showed healing signs of mice skin. So, the MTX-NPs loaded hydrogel can be a promising delivery system against psoriasis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据