期刊
MOLECULAR THERAPY-NUCLEIC ACIDS
卷 26, 期 -, 页码 333-346出版社
CELL PRESS
DOI: 10.1016/j.omtn.2021.07.007
关键词
-
资金
- National Natural Science Foundation of China [81772999, 81974435, 81872409, 819220 52]
- Natural Science Foundation of Guangdong Province [2019B151502011, 2018A030313610]
The study discovered that METTL3 is upregulated in esophageal cancer and promotes tumor progression, being associated with poor prognosis. High METTL3 expression can promote esophageal cancer formation by activating the Notch signaling pathway. The findings provide a molecular basis for the diagnosis and treatment of esophageal cancer.
Esophageal cancer is a lethal malignancy with a high mortality rate, while the molecular mechanisms underlying esophageal cancer pathogenesis are still poorly understood. Here, we found that the N6-methyladenosine (m6A) methyltransferase-like 3 (METTL3) is significantly upregulated in esophageal squamous cell carcinoma (ESCC) and associated with poor patient prognosis. Depletion of METTL3 results in decreased ESCC growth and progression in vitro and in vivo. We further established ESCC initiation and progression models using Mettl3 conditional knockout mouse and revealed that METTL3-mediated m6A modification promotes ESCC initiation and progression in vivo. Moreover, using METTL3 overexpression ESCC cell model and Mettl3 conditional knockin mouse model, we demonstrated the critical function of METTL3 in promoting ESCC tumorigenesis of the Notch signaling pathway. Forced activation of Notch signaling pathway successfully rescues the growth, migration, and invasion capacities of METTL3-depleted ESCC cells. Our data uncovered important mechanistical insights underlying ESCC tumorigenesis and provided molecular basis for the development of novel strategies for ESCC diagnosis and treatment.
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