期刊
MATERIALS HORIZONS
卷 9, 期 2, 页码 694-707出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0mh01982h
关键词
-
资金
- FCT/MEC [UIDB/50011/2020, UIDP/50011/2020, UIDB/50006/2020]
- FEDER under the PT2020 Partnership Agreement [PTDC/BTM-MAT/30869/2017]
- FEDER under BEAT [PTDC/BTM-MAT/30869/2017]
- FCT [PTDC/BTM-MAT/31498/2017]
- [SFRH/BD/143883/2019]
- [2020.01647]
- Fundação para a Ciência e a Tecnologia [PTDC/BTM-MAT/31498/2017] Funding Source: FCT
In this study, a cell culture support system using enzymes immobilized in hydrogels was developed to generate glucose in situ from hydrogel degradation, promoting cell survival and tissue growth. The laminaran-based hydrogels provided glucose for metabolic activity in 3D cell culture, showing high potential in cell survival compared to native cell-laden laminaran hydrogels. This self-feeding hydrogel system opens up possibilities for applications in tissue regeneration, biofactories, disease models, and cell delivery systems.
Hydrogels have been used in combination with cells for several biomedical and biotechnological applications. Nevertheless, the use of bulk hydrogels has exhibited severe limitations in diffusion of oxygen, nutrients, and metabolites. Here, a support for cell culture is reported where glucose is generated in situ by the own hydrogel degradation, allowing cell survival and function while promoting tissue growth. For this purpose, laminaran (or laminarin)-based hydrogels were fabricated, immobilizing the adequate enzymes to obtain structural platforms for 3D cell culture and providing glucose feeding for metabolic activity of cells through polysaccharide degradation. We demonstrate that tumor A549 cells and human mesenchymal stem cells (hMSCs) can use the glucose resultant from the hydrogel degradation to survive and grow in non-added glucose cell culture medium. Additionally, in vivo biocompatibility and biodegradability of laminaran-based hydrogels were explored for the first time. The self-feeding hydrogels exhibited high potential in cell survival compared to native cell-laden laminaran hydrogels over two weeks of sub-cutaneous implantation. Such bioscaffolds with enzyme-empowered degradation capacity can be applied in diverse biotechnological contexts such as tissue regeneration devices, biofactories, disease models, and cell delivery systems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据