期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 176, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/63182
关键词
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资金
- NIH [P40OD018537, T32 GM007133, NS090190, NS102698]
- University of Wisconsin Graduate School
- UW-Madison Women in Science and Engineering Leadership Institute (WISELI)
Adult Drosophila brain can generate new neurons and glia in response to penetrating traumatic brain injury, making it a robust model for studying neural regeneration. The powerful genetic tools and rigorous injury protocols available in Drosophila facilitate further studies into the mechanisms underlying neural regeneration.
The molecular and cellular mechanisms underlying neurogenesis in response to disease or injury are not well understood. However, understanding these mechanisms is crucial for developing neural regenerative therapies. Drosophila melanogaster is a leading model for studies of neural development but historically has not been exploited to investigate adult brain regeneration. This is primarily because the adult brain exhibits very low mitotic activity. Nonetheless, penetrating traumatic brain injury (PTBI) to the adult Drosophila central brain triggers the generation of new neurons and new glia. The powerful genetic tools available in Drosophila combined with the simple but rigorous injury protocol described here now make adult Drosophila brain a robust model for neural regeneration research. Provided here are detailed instructions for (1) penetrating injuries to the adult central brain and (2) dissection, immunohistochemistry, and imaging post-injury. These protocols yield highly reproducible results and will facilitate additional studies to dissect mechanisms underlying neural regeneration.
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