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An Inescapable Cat Odor Exposure Protocol for Studying Innate and Contextual Threat Conditioning in Rats

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JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/63078

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This paper describes a methodology to measure defensive behaviors toward both innate and learned aversive stimuli in rats, including avoidance, freezing, and risk assessment. By exposing rats to cat odor under specific experimental conditions, the study investigated the defensive behavioral responses of rats.
Animals respond to threatening situations by exhibiting a number of defensive behaviors, including avoidance, freezing, and risk assessment. An animal model with an ethological approach offers a deeper insight into the biological mechanisms underlying threat responses. This paper describes a methodology to measure defensive behaviors toward both innate and learned aversive stimuli in rats. Animals were individually exposed to predator odor in an inescapable chamber to elicit a measurable, sustained, defensive state. The experimental design involved placing a rat in a familiar chamber for 10 min followed by exposure to cat odor for another 10 min in the same context. The next day, the rats were

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