A Primer for Single-Cell Sequencing in Non-Model Organisms

Single-cell sequencing technologies have led to a revolution in our knowledge of the diversity of cell types, connections between biological levels of organization, and relationships between genotype and phenotype. These advances have mainly come from using model organisms; however, using single-cell sequencing in non-model organisms could enable investigations of questions inaccessible with typical model organisms. This primer describes a general workflow for single-cell sequencing studies and considerations for using non-model organisms (limited to multicellular animals). Importantly, single-cell sequencing, when further applied in non-model organisms, will allow for a deeper understanding of the mechanisms between genotype and phenotype and the basis for biological variation.

Automated summary

Single-cell sequencing has revolutionized our understanding of cell diversity, organizational connections, and genotype-phenotype relationships. While most research has been focused on model organisms, applying this technology to non-model organisms can uncover new insights that are inaccessible using traditional model organisms.