4.6 Article

Long-Term Hypoxia Negatively Influences Ca2+ Signaling in Basilar Arterial Myocytes of Fetal and Adult Sheep

期刊

FRONTIERS IN PHYSIOLOGY
卷 12, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fphys.2021.760176

关键词

arterial myocytes; calcium oscillations in living cells; calcium sparks; ontogeny; high altitude

资金

  1. National Science Foundation under Major Research Instrumentation, Division of Biological Infrastructure [0923559]
  2. National Institutes of Health Grants [HD083132, HL149608]
  3. Direct For Biological Sciences
  4. Div Of Biological Infrastructure [923559] Funding Source: National Science Foundation

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The regulation of cerebral blood flow is essential for the cerebral arterial vasoreactivity. This study investigates the impact of age and long-term hypoxia on membrane depolarization and intracellular calcium signaling in cerebral arteries using fluorescence imaging techniques. The results show that both altitude and age play a role in affecting the whole-cell and localized calcium signaling, which are important factors for arterial vasoreactivity and cerebral blood flow.
Cerebral arterial vasoreactivity is vital to the regulation of cerebral blood flow. Depolarization of arterial myocytes elicits whole-cell Ca2+ oscillations as well as subcellular Ca2+ sparks due to activation of ryanodine receptors on the sarcoplasmic reticulum. Previous evidence illustrates that contraction of cerebral arteries from sheep and underlying Ca2+ signaling pathways are modified by age and that long-term hypoxia (LTH) causes aberrations in Ca2+ signaling pathways and downstream effectors impacting vasoregulation. We hypothesize that age and LTH affect the influence of membrane depolarization on whole-cell intracellular Ca2+ oscillations and sub-cellular Ca2+ spark activity in cerebral arteries. To test this hypothesis, we examined Ca2+ oscillatory and spark activities using confocal fluorescence imaging techniques of Fluo-4 loaded basilar arterial myocytes of low- and high-altitude term fetal (similar to 145 days of gestation) and adult sheep, where high-altitude pregnant and non-pregnant sheep were placed at 3,801 m for >100 days. Ca2+ oscillations and sparks were recorded using an in situ preparation evaluated in the absence or presence of 30 mM K+ (30K) to depolarize myocytes. Myocytes from adult animals tended to have a lower basal rate of whole-cell Ca2+ oscillatory activity and 30K increased the activity within cells. LTH decreased the ability of myocytes to respond to depolarization independent of age. These observations illustrate that both altitude and age play a role in affecting whole-cell and localized Ca2+ signaling, which are important to arterial vasoreactivity and cerebral blood flow.

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