期刊
FRONTIERS IN PHYSIOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphys.2021.757565
关键词
organoid; 3D printing; testis; spermatogonial stem cells; germline stem cells; tubulogenesis; in vitro spermatogenesis
类别
Short-term germ cell survival and central tissue degeneration pose limitations on organoid cultures. Testicular organoids generated from different mouse strains in 3D printed scaffolds show long-term survival and differentiation capability, but further optimization of culture conditions is needed for sustained germ cell survival, especially in chimeric organoids containing germline stem cells.
Short-term germ cell survival and central tissue degeneration limit organoid cultures. Here, testicular organoids (TOs) were generated from two different mouse strains in 3D printed one-layer scaffolds (1LS) at the air-medium interface displaying tubule-like structures and Leydig cell functionality supporting long-term survival and differentiation of germ cells to the meiotic phase. Chimeric TOs, consisting of a mixture of primary testicular cells and EGFP(+) germline stem (GS) cells, were cultured in two-layer scaffolds (2LSs) for better entrapment. They showed an improved spheroidal morphology consisting of one intact tubule-like structure and surrounding interstitium, representing the functional unit of a testis. However, GS cells did not survive long-term culture. Consequently, further optimization of the culture medium is required to enhance the maintenance and differentiation of germ cells. The opportunities TOs offer to manipulate somatic and germ cells are essential for the study of male infertility and the search for potential therapies.
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