期刊
FRONTIERS IN PHARMACOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphar.2021.781282
关键词
stathmin; microtubule; migration; proliferation; lipopolysaccharide
资金
- National Natural Science Foundation of China [81773348]
- Military Medical Innovation Program in Daping Hospital [2019CXJSC014]
This study reveals an important role of stathmin in the migration and proliferation of dermal fibroblasts. Stathmin is shown to increase with p38/MAPK pathway activation in response to lipopolysaccharide stimulation, resulting in microtubule depolymerization and increased migration and proliferation of dermal fibroblasts. Targeting stathmin or the p38/MAPK pathway could be a potential strategy for clinical interventions in wound healing.
The dermal fibroblast is a crucial executor involved in wound healing, and lipopolysaccharide is a key factor in initiating the migration and proliferation of the dermal fibroblasts, followed by wound healing. However, the underlying molecular mechanism is still unknown. In this study, we demonstrated that stathmin increased concomitantly with p38/MAPK pathway activation by lipopolysaccharide stimulation of the human dermal fibroblast (HDF), which induced microtubule (MT) depolymerization followed by increased HDF migration and proliferation. In contrast, the application of taxol, the small interfering RNA transfection of stathmin, or the application of the p38/MAPK inhibitor SB203580 suppressed MT depolymerization and HDF migration and proliferation. Additionally, the overexpression of a MKK6(Glu) mutant, which constitutively activated p38/MAPK, resulted in MT depolymerization and, subsequently, promoted HDF migration and proliferation. Our data reveal a crucial role of stathmin in HDF migration and proliferation. These findings will provide new targets and strategies for clinical interventions in wound healing.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据