4.7 Article

Tetramethylpyrazine Alleviates Endothelial Glycocalyx Degradation and Promotes Glycocalyx Restoration via TLR4/NF-κB/HPSE1 Signaling Pathway During Inflammation

期刊

FRONTIERS IN PHARMACOLOGY
卷 12, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fphar.2021.791841

关键词

endothelial glycocalyx; TMP; HPSE1; TLR4/NF-kappa B signaling pathway; LPS

资金

  1. National Natural Science Foundation of China [81800389]
  2. Chongqing Technology Innovation and Application Development Special Project [cstc2020jscx-msxmX0070]
  3. Science and Technology Research Program of Chongqing Municipal Education Commission [KJQN202100430]
  4. Chongqing Medical University Scientific Research Cultivating Fund [YXY2020XSZ01]

向作者/读者索取更多资源

This study investigated the molecular mechanism of tetramethylpyrazine (TMP) on vascular endothelial glycocalyx in an inflammatory model. The results showed that TMP protected endothelial glycocalyx integrity by inhibiting its degradation and monocyte adhesion to the endothelium. This protective effect was mediated through the TLR4/NF-kappa B/HPSE1 signaling pathway. TMP could be considered as a novel strategy for protecting endothelial glycocalyx and potentially preventing atherosclerosis.
Tetramethylpyrazine (TMP), a Chinese traditional herbal extraction widely used in treating cardiovascular diseases, could attenuate vascular endothelial injuries, but the underlying mechanism remains incomprehensive. Vascular glycocalyx coating on the endothelium would be damaged and caused endothelial dysfunction in the inflammatory microenvironment, which was the initial factor of morbidity of many vascular diseases, such as atherosclerosis (AS). Here, we thoroughly investigated the molecular mechanism of TMP on vascular endothelial glycocalyx in the LPS-induced inflammatory model both in vitro and in vivo. Results showed that pretreatment with TMP significantly inhibited glycocalyx degradation and monocytes adhesion to the endothelial process. Moreover, TMP pretreatment inhibited the expression of HPSE1 (a major degrading enzyme of endothelial glycocalyx), Toll-like receptor 4 (TLR4), and the translocation of nuclear factor kappa B p65 (NF-kappa B p65). We were utilized withTLR4 siRNA, NF-kappa B inhibitor, and HPSE1 overexpression analysis confirmed TMP's protection on endothelial glycocalyx injury, which further contributed to the monocyte-endothelial adhesion process. It was indicated that TMP might suppress glycocalyx degradation through TLR4/NF-kappa B/HPSE1 signaling pathway. Taken together, our results enriched the occurrence molecular mechanism of glycocalyx shedding and molecular regulation mechanism of TMP in protecting integrity of the glycocalyx structure during inflammation. As TMP is currently used in clinical applications, it may be considered a novel strategy against atherosclerosis through its ability to protect endothelial glycocalyx.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据