期刊
CATALYSTS
卷 11, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/catal11111274
关键词
laccase; ionic liquid; surfactant; pretreatment; biomass; delignification
资金
- Ministry of Science and Technology of Taiwan [109-2221-E-110-020-MY2, 109-2221-E-110-021, 110-2221-E-110-037]
This study aimed to enhance the biodelignification pretreatment of rice straw using laccase in the presence of ionic liquid or surfactant. The addition of [AMIM]Cl and TritonX-100 significantly increased the lignin removal compared to laccase only. The enzymatic saccharification efficiency of rice straw was improved by laccase pretreatment with ionic liquid or surfactant in a single system.
Fungal delignification can be a feasible process to pretreat biomass for bioethanol production if its performance is improved in terms of efficiency through a few modifications. The aim of this study was to enhance the biodelignification pretreatment of rice straw using laccase in the presence of ionic liquid (1-Allyl-3-methylimidazolium chloride, [AMIM]Cl) or surfactant (TritonX-100). Addition of 750 mg/L [AMIM]Cl and 500 mg/L TritonX-100 increases the lignin removal to 18.49% and 31.79%, which is higher than that of laccase only (11.97%). The enzymatic saccharification process was carried out based on different strategies. The highest cellulose conversion, 40.96%, 38.24%, and 37.91%, was obtained after 72 h of enzymatic saccharification when the substrate was washed with distilled water after pretreatment of rice straw with laccase + TritonX-100, laccase + [AMIM]Cl, and laccase only, respectively. In addition, the morphology and structure changes of pretreated and untreated rice straw were studied. Both surface area and cellulose crystallinity are substantially altered after laccase + [AMIM]Cl and laccase + TritonX-100 pretreatment. Enhanced saccharification efficiency of rice straw was achieved by laccase pretreatment with ionic liquid or surfactant in a single system.
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