期刊
BIOLOGY OPEN
卷 10, 期 12, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/bio.059013
关键词
Mouse zygote; Morphokinetics; Shape cycles; Pronuclear fading; Human; Cytoplasm vortices
类别
资金
- Polish Academy Science Centre [UMO-2017/27/B/NZ5/00405]
Morphokinetics of mouse zygotes were studied at different stages, revealing changing patterns of shape and area, as well as the development of vortices during cell division. These shape and area cycles may play a role in promoting exchange with the environment, while the persistent circulation in the cytoplasm could aid in communication between the cell's interior and periphery.
Mouse zygote morphokinetics were measured during interphase, the mitotic period, cytokinesis, and two-cell stage. Sequences of rounder-distorted-rounder shapes were revealed, as were changing patterns of cross section area. A calcium chelator and an actin-disrupting agent inhibited the area changes that occurred between pronuclear envelope breakdown and cytokinesis. During cell division, two vortices developed in each nascent cell and they rotated in opposite directions at each end of the cell, a pattern that sometimes persisted for up to 10 h. Exchange with the environment may have been promoted by these shape and area cycles and persisting circulation in the cytoplasm may have a similar function between a cell's interior and periphery. Some of these movements were sporadically also seen in human zygotes with abnormal numbers of pronuclei and the two-cell stages that developed from these compromised human zygotes.
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