期刊
ADVANCED MATERIALS INTERFACES
卷 9, 期 14, 页码 -出版社
WILEY
DOI: 10.1002/admi.202102046
关键词
antibody response; asymptomatic; COVID-19; early detection; ZnO nanowire
资金
- National Research Council of Science and Technology (NST)
- National Research Foundation (NRF) - Ministry of Science and ICT, Republic of Korea [CRC-16-01-KRICT, NRF-2020M3E9A1043749, NRF2018R1D1A3B07049557]
- Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HI20C0363]
A novel serological assay based on ZnO-NW MP has been developed to detect early antibody responses to SARS-CoV-2 in asymptomatic patients with high sensitivity, enabling accurate diagnosis and estimation of the epidemiology of COVID-19.
A serological immunoassay based on enzyme-linked immunosorbent assay (ELISA) is a crucial tool for screening and identification of human SARS-CoV-2 seroconversion. Various immunoassays are developed to detect the spike 1 (S1) and nucleocapsid (NP) proteins of SARS-CoV-2; however, these serological tests have low sensitivity. Here, a novel microplate (MP) is developed on which a ZnO nanowire (NW) is fabricated by a modified hydrothermal synthesis method. This plate is coated with SARS-CoV-2 NP and used as a fluorescent immunoassay (FIA) to detect antibodies specific for SARS-CoV-2 NP. Compared with the bare MP, the ZnO-NW MP binds high levels (up to 5 mu g mL(-1)) of SARS-CoV-2 NP tagged to histidine without any surface treatment. A novel serological assay based on the ZnO-NW MP is more sensitive than a commercial immunoassay, enabling early detection (within <5 days of a reverse transcription polymerase chain reaction-confirmed COVID-19 infection) of anti-SARS-CoV-2 NP IgG antibodies in asymptomatic patients with COVID-19. This is the first assay to detect early antibody responses to SARS-CoV-2 in asymptomatic patients. Therefore, this serological assay will facilitate accurate diagnosis of COVID-19, as well as estimation of COVID-19 prevalence and incidence.
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