期刊
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
卷 28, 期 10, 页码 5738-5744出版社
ELSEVIER
DOI: 10.1016/j.sjbs.2021.06.003
关键词
Oryza sativa; Callus induction; Shoot regeneration; Rooting; Solidifying agents
类别
资金
- National Research Centre, Cairo, Egypt [10070211]
Plant tissue culture technology provides a solution for meeting the commercial demand on economically important plants like rice, but the choice of gelling agents can significantly impact rice regeneration efficiency. The study showed that different gelling agents have varying effects on rice regeneration, with Gellan gum (Gelrite and Phytagel) performing superiorly in callus induction.
Plant tissue culture technology offers a solution for meeting the increasing commercial demand on economically important plants such as rice, a widespread dietary staple. However, significant genotypespecific morphogenetic responses constitute a considerable on rice regeneration in plant biotechnology contexts. Aside from genotype dependency, the components of the nutrient media including gelling agents have an important impact on regeneration efficiency. The current study explores the effect of different gelling agents on various stages of rice regeneration in two Egyptian rice cultivars-Sakha104 and Giza178. Media solidified with varying concentrations of a variety of gelling agents (agar, bacto agar, gelrite and phytagel) were tested for their impact on the frequency of callus induction, shoot regeneration and rooting. The results indicated gellan gum (gelrite and phytagel) was superior to agar products (agar and bacto agar) for callus induction. By contrast, no significant differences were found between different gelling agents for shoot regeneration. Gellan gum and media solidified with bacto agar were found to lead to significantly higher root regeneration than agar. The Sakha104 cultivar showed better responses than Giza 178 for callus induction and similar performance to the Giza 178 cultivar for root regeneration irrespective of the gelling agent. This work provides insights into the impact of different gelling agents on the morphogenetic response of two rice cultivars and can be used to help maximize the frequency of rice regeneration. (c) 2021 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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