期刊
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
卷 29, 期 4, 页码 2148-2162出版社
ELSEVIER
DOI: 10.1016/j.sjbs.2021.11.046
关键词
Medicago sativa L; Root rot diseases; Saponin and lignin content; SSR; SCoT Molecular genetic markers
类别
资金
- Taif University, Taif, Saudi Arabia [TURSP-2020/110]
Fifteen alfalfa populations were tested for resistance to damping-off disease and the results showed that the resistance was associated with saponin and lignin content in the roots. Breeding for resistance and using molecular markers like SSR and SCoT can contribute to genetic diversity and germplasm classification in alfalfa.
Fifteen alfalfa populations were tested for resistance to the seedling damping-off disease sourced by Rhizoctonia solani, Fusarium solani, and Macrophomina phaseolina. In a laboratory experiment, saponin treatment significantly diminished the mycelial growth of the causal fungi of alfalfa damping-off disease. Roots of the fifteen alfalfa populations varied in saponin and lignin content. Selection for the considerably resistant plants leads to the best growth performance, desirable yield, and high nutritive values such as crude protein (CP), crude fier (CF), nitrogen free extract (NFE), ash, and ether extract (EE) contents. For the PCR reaction, 10 SSR pairs of the JESPR series primers and the cDNA-SCoT technique with seven primers were used. SSR and SCoT revealed some unique markers that could be linked to resistance to damping-off disease in alfalfa that appeared in the considerably resistant alfalfa population (the promised pop.). SSR and SCoT markers can be an excellent molecular method for judging genetic diversity and germplasm classification in tetraploid alfalfa. We recommend breeding for saponin concentration in the alfalfa plant may affect resistance to some diseases like root rot and damping-off because saponin might improve plant growth, yield, and nutritional values. (C) 2021 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据