期刊
MICROMACHINES
卷 12, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/mi12111387
关键词
PCB technology; DNA amplification; RPA; microfluidics; microheaters; E. coli ; molecular diagnostics
类别
资金
- European Regional Development Fund of the European Union
- Greek national funds through the Operational Program Competitiveness, Entrepreneurship and Innovation [T1EDK-03565]
PCB technology enables the commercial fabrication of a microfluidic chip for RPA amplification, showing comparable performance to a thermocycler but with significantly lower power consumption. This microchip is intended for seamless integration with biosensors to develop a point-of-care molecular diagnostics platform.
Printed circuit board (PCB) technology has been recently proposed as a convenient platform for seamlessly integrating electronics and microfluidics in the same substrate, thus facilitating the introduction of integrated and low-cost microfluidic devices to the market, thanks to the inherent upscaling potential of the PCB industry. Herein, a microfluidic chip, encompassing on PCB both a meandering microchannel and microheaters to accommodate recombinase polymerase amplification (RPA), is designed and commercially fabricated for the first time on PCB. The developed microchip is validated for RPA-based amplification of two E. coli target genes compared to a conventional thermocycler. The RPA performance of the PCB microchip was found to be well-comparable to that of a thermocycler yet with a remarkably lower power consumption (0.6 W). This microchip is intended for seamless integration with biosensors in the same PCB substrate for the development of a point-of-care (POC) molecular diagnostics platform.
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