4.6 Article

Enhancement of Binding Kinetics on Affinity Substrates Using Asymmetric Electroosmotic Flow on a Sinusoidal Bipolar Electrode

期刊

MICROMACHINES
卷 13, 期 2, 页码 -

出版社

MDPI
DOI: 10.3390/mi13020207

关键词

heterogeneous immunoassay; induced charged electroosmosis; sinusoidal bipolar electrode; microfluidic chip

资金

  1. National Natural Science Foundation of China [62104195]
  2. Guang Dong Basic and Applied Basic Research Foundation [2020A1515110747]
  3. Basic Research Programs of Taicang, 2020 [TC2020JC02]
  4. Fundamental Research Funds for the Central Universities [D5000210625, G2021KY05101]
  5. Industrial Development and Foster Project of Yangtze River Delta Research Institute of NPU, Taicang [CY20210204]

向作者/读者索取更多资源

In this study, a microfluidic immunosensor with a sinusoidal bipolar electrode is proposed to enhance the rate of antibody binding and improve the detection speed. The results demonstrate the potential of this approach for various immunoassay applications.
In the context of the COVID-19 epidemic, enhancing the transport of analyte to a sensor surface is crucial for rapid detection of biomolecules since common conditions, including low diffusion coefficients, cause inordinately long detection times. Integrated microfluidic immunoassay chips are receiving increasing attention for their low sample volume and fast response time. We herein take advantage of asymmetric ICEO flow at a bipolar sinusoidal electrode to improve the rate of antibody binding to the reaction surface based on finite element modeling. Three different microfluidic cavities are proposed by changing the positions of the surface reaction area. We further investigate the relationship between binding enhancement and reaction surface positions, Damkohler number, and the voltage and frequency of the AC signal applied to the driving electrodes. Furthermore, the influence of the AC signal applied to the sinusoidal bipolar electrode on antigen-antibody-binding performance is studied in detail. Above all, the simulation results demonstrate that the microfluidic immune-sensor with a sinusoidal bipolar electrode could not only significantly improve the heterogeneous immunoassays but also enable efficient enhancement of assays in a selected reaction region within the micro-cavity, providing a promising approach to a variety of immunoassay applications, such as medical diagnostics and environmental and food monitoring.

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