4.6 Article

A Portable Device for Simple Exosome Separation from Biological Samples

期刊

MICROMACHINES
卷 12, 期 10, 页码 -

出版社

MDPI
DOI: 10.3390/mi12101182

关键词

exosome; separation; chitosan scaffold

资金

  1. National Key R&D Program of China [2017YFB0405404]
  2. Strategic Priority Research Program of the Chinese Academy of Sciences [XDA16020900, XDB29050301, XDB32030200]
  3. National Nature Science Foundation of China [31971373, 81803492]
  4. Yunnan Key Research and Development Program [202003AD150009]
  5. China Postdoctoral Science Foundation [2019M660065]
  6. Innovation Program of Science and Research from the DICP, CAS [DICP I201934]

向作者/读者索取更多资源

A new strategy utilizing chitosan electrostatic-adsorption, scaffold substrates, and shuttle flow was proposed for isolating circulating exosomes in a portable device, achieving a recovery rate of over 80% within 20 minutes. The method outperforms traditional ultracentrifugation and allows for in situ lysis and further analysis of RNA and proteins from the exosomes isolated from samples.
Exosomes are membrane-bound nanovesicles secreted by most types of cells, which contain a series of biologically important molecules, such as miRNAs, proteins, and lipids, etc. Emerging evidence show that exosomes can affect the physiological status of cells and are involved in various pathological processes. However, due to their small size and density close to body fluids, it is challenging to separate exosomes from a small volume of biological samples in a simple manner. Herein, we propose a new strategy for isolating circulating exosomes from biological samples in a portable device. This method synergistically integrates chitosan electrostatic-adsorption, scaffold substrates, and shuttle flow to enable the highly effective capture of circulating exosomes with a recovery rate of over 80% within 20 min, which is much better than the performance of traditional ultracentrifugation (5-25%, 3 h). Besides, the isolated exosomes from samples could be lysed in situ and further subjected to RNA concentration detection and protein analysis. In particular, all the necessary procedures for exosome separation could be integrated into a single device without the need for bulky equipment. This established device is portable and easy to operate, which provides a promising platform for the study of exosome biology and clinical diagnosis.

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