期刊
JOURNAL OF NANOBIOTECHNOLOGY
卷 19, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12951-021-01121-x
关键词
Prostate specific antigen; Organic electrochemical transistor; Multiplexed spiral; Biomarker
资金
- Shanghai Municipal Health Commission [202040113]
- Outstanding Young Medical Talent Training Program of Pudong Health Bureau of Shanghai [PWRq2020-40]
- National Natural Science Foundation of China [81901747]
The study developed an interdigitated spiral-based MXene-assisted organic electrochemical transistor biosensor for highly sensitive determination of fPSA/tPSA, demonstrating potential applications in clinical settings.
Background: The ratio of fPSA/tPSA in the grey zone of tPSA with the concentration range between 4 ng/ml and 10 ng/ml is significant for diagnosis of prostate cancer, and highly efficiency quantification of the ratio of fPSA/tPSA remain elusive mainly because of their extremely low concentration in patients' peripheral blood with high biosample complexity. Methods: We presented an interdigitated spiral-based MXene-assisted organic electrochemical transistors (isMOECTs) biosensor for highly sensitive determination of fPSA/tPSA. The combination of MXene and the interdigitated multiple spiral architecture synergistically assisted the amplification of amperometric signal of biosensor with dual functionalizations of anti-tPSA and anti-fPSA. Results: The ultrasensitivity of the biosensor was enhanced by tunable multiple spiral architecture and MXene nanomaterials; and the sensor exhibited improved detection limit of tPSA and fPSA down to 0.01 pg/ml and acceptable performance of selectivity, repeatability and stability. Moreover, the isMOECTs displayed area under the curve (AUC) value of 0.8138, confirming the potential applications of isMOECTs in clinics. Conclusions: The merits of isMOECTs biosensor demonstrated the reliability of MXene-assisted organic electrochemical transistor biosensor with multiple interdigitated spiral for ultrasensitive quantification of fPSA/tPSA, suggesting potential current and future point-of-care testing applications.
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