4.6 Article

A single dual-targeting fluorescent probe enables exploration of the correlation between the plasma membrane and lysosomes

期刊

JOURNAL OF MATERIALS CHEMISTRY B
卷 10, 期 4, 页码 582-588

出版社

ROYAL SOC CHEMISTRY
DOI: 10.1039/d1tb02200h

关键词

-

资金

  1. Natural Science Foundation of Shandong Province, China [ZR2020QB101, ZR2021QE252, ZR2019BEM015, ZR2019MEM034, ZR2019PB029]
  2. Postdoctoral Innovation Project of Shandong Province [202102060]
  3. Introduction and Cultivation Plan of Young Innovative Talents in Colleges and Universities of Shandong Province

向作者/读者索取更多资源

The interactions between lysosomes and the plasma membrane play a crucial role in maintaining normal cell activity, and a newly constructed dual-targeting probe, Mem-Lyso, allows for simultaneous visualization of these organelles for the first time. Through its ability to track and analyze dynamic changes in different bioprocesses, Mem-Lyso revealed noticeable fusion between the plasma membrane and lysosomes during apoptosis, while also distinguishing cholesterol content in the plasma membrane, providing a potential tool for biological research and disease diagnosis.
The interactions between organelles can maintain normal cell activity. Lysosomes, as waste disposal systems of cells, have many important interactions with the plasma membrane, especially in the repair of cracked plasma membrane. Unfortunately, a way to study the relationship between them synchronously is still lacking. Therefore, in this work, we constructed a dual-targeting probe (Mem-Lyso) to simultaneously visualize the plasma membrane and lysosomes for the first time. Taking advantage of dual-targeting, the probe Mem-Lyso could successfully track and analyze the dynamic changes of the plasma membrane and lysosomes in different bioprocesses. The experimental results demonstrated that, compared to the normal status, there was obvious fusion between the plasma membrane and lysosomes in the apoptosis process. Furthermore, because of the sensitivity to polarity, Mem-Lyso could label the plasma membrane and lysosomes with red and yellow colors in cells, respectively. Moreover, the skeleton and gastrointestinal wall of zebrafish were visualized by dual-color imaging, respectively. More importantly, the dual-targeting property endowed Mem-Lyso with the ability to spatially distinguish the cholesterol (CL) content in the plasma membrane, which provided a potential detection tool for biological research and diagnosis of related diseases.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据