期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 10, 期 9, 页码 1386-1392出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1tb02557k
关键词
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资金
- Chinese Government [2017YFE0132300, ACSRF65827]
- Australian Government [2017YFE0132300, ACSRF65827]
- National Natural Science Foundation of China [NSFC 51720105015, 51922097, 51929201, 51772124, 51872282]
- CAS-Croucher Funding Scheme for Joint Laboratories [CAS18204]
In this study, we successfully synthesized core-shell quantum dot nanocrystals and magnetic nanoparticles, and applied the ctDNA-triggered hybridization chain reaction for the detection of CYFRA21-1 DNA associated with lung cancer. Compared to conventional methods, this new approach offers improved sensitivity and accuracy.
Sensitive detection of circulating tumor DNA (ctDNA) in vitro has attracted growing attention owing to its potential application in diagnostics of cancer. In this study, we synthesized hydrophilic AgInS2@ZnS core-shell quantum dot nanocrystals and magnetic Fe3O4 nanoparticles, and then the ctDNA triggered hybridization chain reaction was used to detect the CYFRA21-1 DNA associated with lung cancer. In the presence of CYFRA21-1 DNA, three hairpin structures were activated to turn on successively, resulting in the accumulation of quantum dots and eliciting considerable changes of the fluorescence signal. Compared with the conventional fluorescence detection, Fe3O4 provides magnetic adsorption properties and a large surface area for immobilizing and aggregating quantum dot nanoparticles attached to single-stranded DNA. The concentration of CYFRA21-1 is closely related to the number of quantum dots remaining after magnetic adsorption, which provides a promising approach for ctDNA quantification.
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