Revisiting Biological Nitrogen Fixation Dynamics in Soybeans

Biological nitrogen (N) fixation is the most relevant process in soybeans (Glycine max L.) to satisfy plant N demand and sustain seed protein formation. Past studies describing N fixation for field-grown soybeans mainly focused on a single point time measurement (mainly toward the end of the season) and on the partial N budget (fixed-N minus seed N removal), overlooking the seasonal pattern of this process. Therefore, this study synthesized field datasets involving multiple temporal measurements during the crop growing season to characterize N fixation dynamics using both fixed-N (kg ha(-1)) and N derived from the atmosphere [Ndfa (%)] to define: (i) time to the maximum rate of N fixation (beta(2)), (ii) time to the maximum Ndfa (alpha(2)), and (iii) the cumulative fixed-N. The main outcomes of this study are that (1) the maximum rate of N fixation was around the beginning of pod formation (R3 stage), (2) time to the maximum Ndfa (%) was after full pod formation (R4), and (3) cumulative fixation was positively associated with the seasonal vapor-pressure deficit (VPD) and growth cycle length but negatively associated with soil clay content, and (4) time to the maximum N fixation rate (beta(2)) was positively impacted by season length and negatively impacted by high temperatures during vegetative growth (but positively for VPD, during the same period). Overall, variation in the timing of the maximum rate of N fixation occurred within a much narrower range of growth stages (R3) than the timing of the maximum Ndfa (%), which varied broadly from flowering (R1) to seed filing (R5-R6) depending on the evaluated studies. From a phenotyping standpoint, N fixation determinations after the R4 growth stage would most likely permit capturing both maximum fixed-N rate and maximum Ndfa (%). Further investigations that more closely screen the interplay between N fixation with soil-plant-environment factors should be pursued.

Automated summary

This study synthesized field datasets on soybean nitrogen fixation, finding that the maximum nitrogen fixation rate occurs around the beginning of pod formation, while the maximum Ndfa % occurs after full pod formation. The cumulative fixation is influenced by seasonal vapor-pressure deficit and growth cycle length, with the maximum nitrogen fixation rate time being impacted by season length and temperature during vegetative growth. Variations in the timing of nitrogen fixation were observed within a narrower range of growth stages compared to Ndfa %.