期刊
FRONTIERS IN PLANT SCIENCE
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2021.754537
关键词
Vitis vinifera; raffinose biosynthesis; RFOs sugars; cold acclimation; woody tissues
资金
- FCT-Portuguese Foundation for Science and Technology [UIDB/04050/2020]
- FCT [SFRH/BD/135782/2018, SFRH/BPD/115518/2016]
- European Funds [FEDER/POCI/COMPETE2020, PTDC/BIAFBT/30341/2017, POCI-01-0145-FEDER-030341, PTDC/BIA-FBT/28165/2017, POCI-01-0145-FEDER-028165, NORTE-01-0145-FEDER-000041]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/115518/2016, SFRH/BD/135782/2018] Funding Source: FCT
The accumulation of raffinose family oligosaccharides (RFOs) is a critical response of plants to abiotic stresses, including cold. This study investigated the role of galactinol synthases (GolS) and raffinose synthases (RafS) in the cold response of grapevine woody tissues. Results showed that cold acclimation led to an increase in sugar content and abscisic acid (ABA) levels, and VviRafS5 was identified as a key player in grapevine cold response. The study suggests that ABA accumulation upregulates VivRafS5, leading to raffinose synthesis.
The accumulation of raffinose family oligosaccharides (RFOs) is a hallmark of plant response to different abiotic stresses, including cold. The synthesis of galactinol, by galactinol synthases (GolS), and raffinose, by raffinose synthases (RafS), are fundamental for stress-induced accumulation of RFOs, but the role of these enzymes in the cold response of grapevine (Vitis vinifera L.) woody tissues is still unclear. To address this gap in the literature, 1-year-lignified grapevine canes were incubated at 4 degrees C for 7 and 14 days and tissues were analyzed for sugar content and gene expression. Results showed that, in parallel to starch breakdown, there was an increase in soluble sugars, including sucrose, glucose, fructose, raffinose, and stachyose. Remarkably, abscisic acid (ABA) levels increased during cold acclimation, which correlated with the increased expression of the key ABA-synthesis genes VviNCED2 and VviNCED3. Expression analysis of the VviGolS and VviRafS family allowed the identification of VviRafS5 as a key player in grapevine cold response. The overexpression of VviRafS5 in Saccharomyces cerevisiae allowed the biochemical characterization of the encoded protein as a raffinose synthase with a size of ~87 kDa. In grapevine cultured cells, VviRafS5 was upregulated by cold and ABA but not by heat and salt stresses. Our results suggest that ABA accumulation in woody tissues during cold acclimation upregulates VivRafS5 leading to raffinose synthesis.
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