Transcriptome Co-expression Network and Metabolome Analysis Identifies Key Genes and Regulators of Proanthocyanidins Biosynthesis in Brown Cotton

Brown cotton fiber (BCF) is a unique raw material of naturally colored cotton (NCC). But characteristics of the regulatory gene network and metabolic components related to the proanthocyanidins biosynthesis pathway at various stages of its fiber development remain unclear. Here, the dynamic changes in proanthocyanidins biosynthesis components and transcripts in the BCF variety Zong 1-61 and its white near-isogenic lines (NILs) RT were characterized at five fiber developmental stages (0, 5, 10, 15, and 20 days post-anthesis; DPA). Enrichment analysis of differentially expressed genes (DEGs), comparison of metabolome differences, and pathway enrichment analysis of a weighted gene correlation network analysis together revealed the dominant gene expression of flavonoid biosynthesis (FB), phenylpropanoid metabolisms, and some carbohydrate metabolisms at 15 or 20 DPA than white cotton. Eventually, 63 genes were identified from five modules putatively related to FB. Three R2R3-MYB and two bHLH transcription factors were predicted as the core genes. Further, GhANS, GhANR1, and GhUFGT2 were preliminarily regulated by GhMYB46, GhMYB6, and GhMYB3, respectively, according to yeast one-hybrid assays in vitro. Our findings provide an important transcriptional regulatory network of proanthocyanidins biosynthesis pathway and dynamic flavonoid metabolism profiles.

Automated summary

This study characterized the changes in proanthocyanidins biosynthesis components and transcripts in brown cotton fiber (BCF) variety Zong 1-61 at different stages of fiber development. The results revealed that flavonoid biosynthesis, phenylpropanoid metabolisms, and carbohydrate metabolisms dominated gene expression at 15 or 20 DPA. 63 genes, including three core genes, were identified as potentially related to flavonoid biosynthesis. Yeast one-hybrid assays confirmed the regulation of GhANS, GhANR1, and GhUFGT2 by GhMYB46, GhMYB6, and GhMYB3, respectively.