4.7 Article

GhCLCg-1, a Vacuolar Chloride Channel, Contributes to Salt Tolerance by Regulating Ion Accumulation in Upland Cotton

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FRONTIERS IN PLANT SCIENCE
卷 12, 期 -, 页码 -

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FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2021.765173

关键词

chloride channel; Gossypium hirsutum; vacuolar; ion content; salt tolerance

资金

  1. National Natural Science Foundation of China [32001596]
  2. National Key Research and Development Program for Crop Breeding [2018YFD0100306]

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Soil and freshwater salinization is becoming a global issue, affecting plant growth negatively. Research on chloride ion (Cl-) stress is limited compared to sodium ion (Na+) stress. A chloride channel gene GhCLCg-1 was identified in upland cotton, regulating ion accumulation and enhancing salt tolerance in plants.
Soil and freshwater salinization is increasingly becoming a problem worldwide and has adversely affected plant growth. However, most of the related studies have focused on sodium ion (Na+) stress, with relatively little research on chloride ion (Cl-) stress. Here, we found that upland cotton (Gossypium hirsutum) plants accumulated Cl- and exhibited strong growth inhibition under NaCl or KCl treatment. Then, a chloride channel gene (GhCLCg-1) was cloned from upland cotton. Phylogenetic and sequence analyses indicated that GhCLCg-1 was highly homologous to AtCLCg and also have conserved voltage_CLC and CBS domains. The subcellular localization assay showed that GhCLCg-1 was localized on the vacuolar membrane. Gene expression analyses revealed that the expression of GhCLCg-1 increased rapidly in cotton in response to chloride stress (NaCl or KCl), and the transcript levels increased as the chloride stress intensified. The overexpression of GhCLCg-1 in Arabidopsis thaliana changed the uptake of ions with a decrease of the Na+/K+ ratios in the roots, stems, and leaves, and enhanced salt tolerance. In contrast, silencing GhCLCg-1 in cotton plants increased the Cl- contents in the roots, stems, and leaves and the Na+/K+ ratios in the stems and leaves, resulting in compromised salt tolerance. These results provide important insights into the toxicity of chloride to plants and also indicate that GhCLCg-1 can positively regulates salt tolerance by adjusting ion accumulation in upland cotton.

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