期刊
FRONTIERS IN PLANT SCIENCE
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2021.759623
关键词
clubroot resistance; RNA-seq; phytohormone; metabolome profiling; Brassica rapa L; P; brassicae
资金
- National Science Foundation of China [31801874, 31872945]
- Distinguished Young Scientists from Henan Academy of Agricultural Sciences [2021JQ03]
- Scientific Research Foundation for Returned Scholars from Department of Human Resources and Social Security of Henan Province, Zhongyuan Scholar Program [202101510003]
- Sci-Tech Innovation Team of Henan Academy Agricultural Sciences [2021TD06]
- Fostering Project for Basic Research of Zhengzhou University [JC21310015]
This study investigated the transcriptome and metabolome changes in Brassica crops infected with Plasmodiophora brassicae, highlighting the role of plant hormones in defense mechanisms and resistance to clubroot disease. The findings suggest a general model of hormone-mediated defense mechanism in plants and pave the way for enhancing clubroot resistance in Brassica rapa.
Plasmodiophora brassicae, an obligate biotrophic pathogen-causing clubroot disease, can seriously affect Brassica crops worldwide, especially Chinese cabbage. Understanding the transcriptome and metabolome profiling changes during the infection of P. brassicae will provide key insights in understanding the defense mechanism in Brassica crops. In this study, we estimated the phytohormones using targeted metabolome assays and transcriptomic changes using RNA sequencing (RNA-seq) in the roots of resistant (BrT24) and susceptible (Y510-9) plants at 0, 3, 9, and 20 days after inoculation (DAI) with P. brassicae. Differentially expressed genes (DEGs) in resistant vs. susceptible lines across different time points were identified. The weighted gene co-expression network analysis of the DEGs revealed six pathways including Plant-pathogen interaction and Plant hormone signal transduction and 15 hub genes including pathogenic type III effector avirulence factor gene (RIN4) and auxin-responsive protein (IAA16) to be involved in plants immune response. Inhibition of Indoleacetic acid, cytokinin, jasmonate acid, and salicylic acid contents and changes in related gene expression in R-line may play important roles in regulation of clubroot resistance (CR). Based on the combined metabolome profiling and hormone-related transcriptomic responses, we propose a general model of hormone-mediated defense mechanism. This study definitely enhances our current understanding and paves the way for improving CR in Brassica rapa.
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