期刊
FRONTIERS IN MICROBIOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2021.748594
关键词
ulcerative colitis; Tibetan tea; RNA-seq; gut microbiota; immune response
类别
资金
- Science and Technology Department of Sichuan Province [2020YFH0130]
- Talent Introduction Project of Sichuan University of Science Engineering [2019RC29]
- Academician (Expert) Workstation Project of Sichuan University of Science Engineering [2018YSCZZ01]
- Innovation and Entrepreneurship Program for College Students [S201910622061, cx2020109]
This study reveals the protective impact of Tibetan tea extract (TTE) on colitis by reducing inflammation, improving tissue damage, and promoting the growth of beneficial microbes. The transcriptome analysis suggests that the positive effect of TTE is achieved through changes in gene expression related to signaling pathways and the immune system.
Traditionally, Ya'an Tibetan tea is routinely consumed by local people in the Tibet region. It is believed to possess promising anti-inflammatory benefits. This study was conducted to elucidate the protective impact of Tibetan tea extract (TTE) on dextran sodium sulfate (DSS)-induced colitis in mice. Mice were split into four groups: control (C) group, Tibetan tea (T) group, DSS-induced model (CD) group, and Tibetan tea + DSS (TD) group. The intake of TTE significantly reduced the clinical symptoms of ulcerative colitis (UC) by alleviating the impact of cellular damage and reducing glandular hypertrophy and the infiltration of inflammatory cells. UC led to a prominent shift of the microbial communities in the gut. Interestingly, the beneficial microbes, such as Lactobacillus reuteri, Bifidobacterium choerinum, and Lactobacillus intestinalis, were significantly increased in TTE-treated mice when compared to any other experimental group. The transcriptome analysis revealed that the positive effect of TTE on UC could be attributed to changes in the G alpha (i) signaling pathway and the innate immune system. The genes related to inflammation and immune system pathways were differentially expressed in the TTE-treated group. Moreover, the relative expression of genes linked to the inflammatory TLR4/MyD88/NF-kappa B signaling pathway was significantly downregulated toward the level of normal control samples in the TD group. Overall, this study revealed the modulatory effect by which TTE reversed the development and severity of chronic colon damage.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据