Alteration of Porcine Intestinal Microbiota in Response to Dietary Manno-Oligosaccharide Supplementation

Manno-oligosaccharide (MOS) is a prebiotic derived from natural plants or yeasts. Here, we explored the response of intestinal microbiota and epithelial functions after ingestion of MOS in a porcine model. Sixteen pigs were randomly assigned into two treatments and fed with basal or MOS-containing (0.3% MOS) diet for 21 days. Results showed that MOS supplementation increased the cecal acetate content and ileal 16S rRNA gene copies (p < 0.05). Importantly, MOS decreased the abundance of phylum Proteobacteria in cecal digesta (p < 0.05). Moreover, MOS elevated the expression level of SCL5A8 and GPR109A but decreased the expression levels of HDAC1 and TNF-alpha in the ileal and cecal mucosa (p < 0.05). MOS upregulated the expression levels of tight-junction protein (ZO-1, claudin-1, and occludin) and IGF-1 in the ileum and cecum (p < 0.05). This study presents the alteration of intestinal microbiota composition and intestinal barrier function after MOS administration, and facilitates our understanding of the mechanisms behind the dietary MOS-modulated intestinal microbiota and health.

Automated summary

This study examined the effects of Manno-oligosaccharide (MOS) supplementation on intestinal microbiota and epithelial functions in pigs. Results showed that MOS increased acetate content in the cecum and the gene copies of 16S rRNA in the ileum. Additionally, MOS reduced the abundance of Proteobacteria in the cecum digesta. MOS also influenced the expression levels of various proteins and genes associated with intestinal barrier function in the ileum and cecum. The findings contribute to our understanding of how dietary MOS modulates intestinal microbiota and health.