期刊
FRONTIERS IN MICROBIOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2021.796715
关键词
helper plasmid; ISEcp1; IS15DI; polymorphism; plasmid fusion
类别
资金
- National Key R&D Program of China [2018YFD0500300]
- Basic Research Fund of Shenzhen [20170410160041091]
- Collaborative Research Fund from the Research Grant Council of the Government of Hong Kong SAR
- Research Impact Fund [R5011-18F]
This study investigates the molecular mechanisms underlying the plasmid fusion process mediated by insertion sequence (IS) elements, by monitoring the interactions between a helper plasmid and two common mobile genetic elements (MGEs). The use of MinION single-molecule long-read sequencing technology provides valuable information on genetic polymorphism and plasmid heterogeneity in different multidrug resistance (MDR) encoding bacteria.
We recently reported the recovery of a novel IncI1 type conjugative helper plasmid which could target mobile genetic elements (MGE) located in non-conjugative plasmid and form a fusion conjugative plasmid to mediate the horizontal transfer of the non-conjugative plasmid. In this study, interactions between the helper plasmid pSa42-91k and two common MGEs, ISEcp1 and IS15DI, which were cloned into a pBackZero-T vector, were monitored during the conjugation process to depict the molecular mechanisms underlying the plasmid fusion process mediated by insertion sequence (IS) elements. The MinION single-molecule long-read sequencing technology can dynamically reveal the plasmid recombination events and produce valuable information on genetic polymorphism and plasmid heterogeneity in different multidrug resistance (MDR) encoding bacteria. Such data would facilitate the development of new strategies to control evolution and dissemination of MDR plasmids.
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