期刊
FRONTIERS IN MICROBIOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2021.802926
关键词
biomineralization; bioremediation; fungal biomass; uranium; waste water; Penicillium simplicissimum
类别
资金
- Bundesministerium fur Bildung und Forschung (BMBF) [02NUK030 F]
- German Federal Ministry of Education and Research (BMBF) [03SF0451]
- Talent Acquisition Program (Programa de Captacion de Talento en Grados Universitarios) - University of Granada (Spain)
Remediation of heavy-metal-contaminated sites is a serious global environmental issue, with bioremediation being a more eco-friendly and cost-effective alternative. The fungus Penicillium simplicissimum KS1, isolated from a flooding water area of a former uranium mine, shows potential in uranium bioremediation through biomineralization.
The remediation of heavy-metal-contaminated sites represents a serious environmental problem worldwide. Currently, cost- and time-intensive chemical treatments are usually performed. Bioremediation by heavy-metal-tolerant microorganisms is considered a more eco-friendly and comparatively cheap alternative. The fungus Penicillium simplicissimum KS1, isolated from the flooding water of a former uranium (U) mine in Germany, shows promising U bioremediation potential mainly through biomineralization. The adaption of P. simplicissimum KS1 to heavy-metal-contaminated sites is indicated by an increased U removal capacity of up to 550 mg U per g dry biomass, compared to the non-heavy-metal-exposed P. simplicissimum reference strain DSM 62867 (200 mg U per g dry biomass). In addition, the effect of temperature and cell viability of P. simplicissimum KS1 on U biomineralization was investigated. While viable cells at 30 & DEG;C removed U mainly extracellularly via metabolism-dependent biomineralization, a decrease in temperature to 4 & DEG;C or use of dead-autoclaved cells at 30 & DEG;C revealed increased occurrence of passive biosorption and bioaccumulation, as confirmed by scanning transmission electron microscopy. The precipitated U species were assigned to uranyl phosphates with a structure similar to that of autunite, via cryo-time-resolved laser fluorescence spectroscopy. The major involvement of phosphates in U precipitation by P. simplicissimum KS1 was additionally supported by the observation of increased phosphatase activity for viable cells at 30 & DEG;C. Furthermore, viable cells actively secreted small molecules, most likely phosphorylated amino acids, which interacted with U in the supernatant and were not detected in experiments with dead-autoclaved cells. Our study provides new insights into the influence of temperature and cell viability on U phosphate biomineralization by fungi, and furthermore highlight the potential use of P. simplicissimum KS1 particularly for U bioremediation purposes.Graphical Abstract
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