4.7 Review

Preclinical Animal Models for Q Fever Vaccine Development

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Article Immunology

A Novel Marmoset (Callithrix jacchus) Model of Human Inhalational Q Fever

Michelle Nelson et al.

Summary: Common marmosets were susceptible to inhalational infection with Coxiella burnetii in a dose-dependent manner, producing a disease similar to human Q fever. The illness was characterized by febrile response, weight loss, liver enzyme dysfunction, circulating INF-gamma and bacteraemia. Histological evidence showed macrophage and lymphocyte infiltration into the lung resulting in granulomatous alveolitis.

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The Feasibility of Using Coxiella burnetii Avirulent Nine Mile Phase II Viable Bacteria as a Live Attenuated Vaccine Against Q fever

Venkatesh Kumaresan et al.

Summary: This study demonstrates that using viable NMII as a live attenuated vaccine can confer long-term protection against virulent C. burnetii infection. Intranasal immunization shows higher level of protection compared to other routes.

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Q Fever Vaccine Development: Current Strategies and Future Considerations

Carrie Mae Long

Summary: Q fever is a zoonotic disease caused by the intracellular pathogen Coxiella burnetii, with only one commercial vaccine available, limited to use in Australia due to potential hypersensitivity reactions. Pre-clinical research is a major focus for developing improved Q fever vaccines.

PATHOGENS (2021)

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Subunit Vaccines Using TLR Triagonist Combination Adjuvants Provide Protection Against Coxiella burnetii While Minimizing Reactogenic Responses

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Summary: Q fever, caused by Coxiella burnetii, poses a potential bioterrorism threat due to its transmission route and resistance to disinfectants. The only licensed vaccine, Q-VAX, can cause severe reactions in sensitized individuals, leading to regulatory reluctance. Research on subunit vaccines with TLR agonists shows potential for protective immunity with reduced reactogenicity against C. burnetii infection.

FRONTIERS IN IMMUNOLOGY (2021)

Article Immunology

Contributions of lipopolysaccharide and the type IVB secretion system to Coxiella burnetii vaccine efficacy and reactogenicity

Carrie M. Long et al.

Summary: The bacterial causative agent of Q fever, Coxiella burnetii, requires the Dot/Icm type IVB secretion system (T4BSS) for full virulence, but not for vaccine-induced protection. The length of lipopolysaccharide (LPS) does not seem to contribute to reactogenicity. Genetically modified C. burnetii strains show promise as improved whole cell vaccines.

NPJ VACCINES (2021)

Article Immunology

Coxiella burnetii Whole Cell Vaccine Produces a Th1 Delayed-Type Hypersensitivity Response in a Novel Sensitized Mouse Model

Alycia P. Fratzke et al.

Summary: Q-VAX (R), a vaccine for Q fever, provides long-term protection but can cause reactions in sensitized individuals, limiting its use outside Australia. Studying different mouse strains revealed that the reactions to C. burnetii whole cell vaccine are consistent with a Th1 delayed-type hypersensitivity response.

FRONTIERS IN IMMUNOLOGY (2021)

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Sex Disparities in Efficacy in COVID-19 Vaccines: A Systematic Review and Meta-Analysis

Alessia Bignucolo et al.

Summary: A systematic review and meta-analysis on COVID-19 vaccines investigated the influence of sex on vaccine efficacy, finding that men had significantly higher efficacy compared to women in the vaccine group, while women showed increased toxicity. These findings highlight the importance of considering sex as a core variable in the design of clinical trials for COVID-19 vaccines.

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Comparative virulence of diverse Coxiella burnetii strains

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Standardized guinea pig model for Q fever vaccine reactogenicity

Laurie A. Baeten et al.

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Biological sex affects vaccine efficacy and protection against influenza in mice

Ashley L. Fink et al.

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Sex differences shape the response to infectious diseases

Molly A. Ingersoll

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David W. C. Beasley et al.

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Early cytokine and antibody responses against Coxiella burnetii in aerosol infection of BALB/c mice

Teske Schoffelen et al.

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE (2015)

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Volker Gerdts et al.

ILAR JOURNAL (2015)

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Immunogenicity of the Q fever skin test

Teske Schoffelen et al.

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Galleria mellonella as an alternative model of Coxiella burnetii infection

I. H. Norville et al.

MICROBIOLOGY-SGM (2014)

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The effect of aging of the immune system on vaccination responses

Janet M. Lord

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Coxiella burnetii infection in C57BL/6 mice aged 1 or 14 months

Marc Leone et al.

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY (2007)

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Clinical and pathologic changes in a guinea pig aerosol challenge model of acute Q fever

K. E. Russell-Lodrigue et al.

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Effect of sex on Coxiella burnetii infection:: Protective role of 17β-estradiol

M Leone et al.

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